Scientist Discovery Biology/ T-cells

Our client, a world leader in life sciences and biotechnology, is
looking for a “Scientist – Discovery Biology/ T cells” based in San
Diego, CA.

Job Duration: Long Term Contract (Possibility of Extension)

Pay Rate: $49.20/hr on W2

Company Benefits: Medical, Paid Sick leave,
401K

Join the Discovery Biology team to support a high-priority binder
screening campaign for T-cell-based therapies. Characterize candidate binders
(scFvs/VHHs) for potency, specificity, affinity, functionality, and safety
through T-cell engineering and functional validation assays.

Key Responsibilities

• Perform non-viral
  engineering of primary human T cells using mRNA or transposon-based DNA
  systems via electroporation/nucleofection; monitor cell viability,
  recovery, and synthetic receptor expression.
  
• Isolate, maintain,
  and expand primary T cells from PBMCs and Jurkat cell lines using aseptic
  techniques.
  
• Execute
  high-throughput binder screening and multi-color flow cytometry (FACS) to
  assess surface expression and phenotypic markers; analyze data using
  FlowJo.
  
• Conduct T-cell
  activation and functional assays, including cytotoxicity assays (IncuCyte
  live-cell imaging/LDH release), cytokine profiling (ELISA/MSD/Luminex),
  and proliferation assays (CFSE).
  
• Record protocols,
  deviations, raw data, and experimental results accurately in an Electronic
  Lab Notebook (ELN) to support data integrity and regulatory filings.
  
• Support screening
  scale-up using liquid-handling robotics, as applicable.
  

Required Qualifications

• Bachelor's or
  Master's degree in Immunology, Molecular Biology, Bioengineering, or a
  related biological science.
  
• Minimum 2 years of
  hands-on wet-lab experience, preferably in an industry setting.
  
• Strong aseptic
  technique and experience isolating primary T cells from PBMCs.
  
• Mandatory
  expertise in flow cytometry acquisition and data analysis.
  

Preferred Qualifications

• Hands-on
  experience with non-viral T-cell transfection/electroporation or
  nucleofection.
  
• Experience with
  liquid-handling robotics and high-throughput screening workflows.
  
• Proficiency in
  FlowJo and functional T-cell assay techniques.
  

If interested, please send us your updated resume at

[email protected]/[email protected]