Lab Biologist

Axle is a bioscience andinformation technology company that offers advancements intranslational research, biomedical informatics, and datascience applications to research centers and healthcareorganizations nationally and abroad. With experts inbiomedical science, software engineering, and programmanagement, we focus on developing and applying researchtools and techniques to empower decision-making andaccelerate research discoveries. We work with some of thetop research organizations and facilities in the countryincluding multiple institutes at the National Institutes ofHealth (NIH).

Axle is seeking a Lab Biologist to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute of Environmental Health Sciences (NIEHS) located in Research Triangle Prk, NC.

Benefits We Offer:

• Paid Time Off and Paid Holidays
• 401K match up to 5%
• EducationalBenefits for Career Growth
• Employee Referral Bonus
• FlexibleSpending Accounts:
  • Healthcare (FSA)
  • ParkingReimbursement Account (PRK)
  • DependentCare Assistant Program (DCAP)
  • TransportationReimbursement Account (TRN)

Overall Position Summary and Objectives The purpose of the position is to work in the laboratory both generating, recording and analyzing data as well as broad management of the lab

• Meet with lab members to present updates - Weekly
• Analyze the experiment results and present findings in project meetings, laboratory and outside meetings. 1
• Present data and results at regularly scheduled lab data clubs and journal clubs. 2
• Prepare posters and oral presentations for delivery at scientific meetings. 3
• Performs research activities and other tasks in a laboratory environment.
• Perform sample preparation for lab assays and for collaboration.
• Perform DNA and RNA extraction and quantification.
• Conduct ELISA for multiple biochemical parameters.
• Perform experiments, including DNA and RNA purification, polymerase chain reactions, real-time PCR, Southern and Northern blotting, cloning of DNA segments and sequencing of DNA.
• Perform cell culture and biochemistry experiments, including basic tissue culture techniques, isolation of proteins from tissues/cells, Western blotting and immunohistochemistry/immunofluorescence.
• Records observations and measurements and reports results at weekly laboratory meetings.
• Analyze experiment results and present findings in project meetings.
• Present and communicate results with Project Team and staff.
• Prepare a weekly report to include: a summary of the samples (experimental or clinical), the modifications measured, and the antibodies validated
• Prepare and deliver periodic oral group meeting reports.
• Documents and analyzes data from experiments.
• Collect, analyze and interpret data for experimental results; prepare tables and figures.
• Perform statistical analysis of the results to ascertain the association or lack thereof of each modification with the particular condition, diagnosis or manipulation of the samples.
• Organize data into tables and figures with Prism Plot or other data analysis/visualization software.
• Analyze the experimental results and organize findings into tables and figures.
• Evaluates new technologies and instruments.
• Collaborate with staff on cutting-edge cell biology and bioassay technologies for the development of disease-relevant assays for screening.
• Maintains lab notebooks and lab equipment.
• Update and maintain records of experiments and results.
• Perform routine laboratory maintenance; ensure research supplies are kept stocked.
• Maintain and update accurate records of daily experiments and results including research documentation.
• Maintain and update laboratory notebooks and equipment.
• Maintain accurate research records digitally.
• Writes technical reports and prepares manuscripts.
• Write and review technical protocols and reports documenting analytical development studies pertaining to assigned work.
• Write experiment reports and prepare manuscripts for publication in collaboration with other investigators.
• Performs lab techniques which study the chemical processes of living organisms.
• Support cell-based projects with tissue culture; cell culture maintenance including stem cells.
• Perform assays to validate antibodies western blot analysis, ELISA and immunoprecipitation.
• Conducts research experiments that will determine the effects of food, drugs, serums, hormones, and other substances on tissues and processes of living organisms.
• Carry out drug repurposing screens against compound collections using the assays developed or transferred from collaborators.
• Set-up and maintain a cell culture lab; provide the material for compound screens.
• Conduct routine and non-routine sample preparation/extraction methods for a variety of biological matrices including but not limited to plasma, urine, skeletal muscle and brain tissue.
• Conduct research experiments that will determine the effects of food, drugs, serums, hormones and other substances on tissues and processes of living organisms.
• Presents findings at scientific conferences and meetings.

1, 2, 3, 4, 5 representspriority rankings, where 1 is highest priority and 5 islowest priority of those ranked

Masters

Additional Qualifications:

Certifications & Licenses

• Good Laboratory Practice (GLP)
• Biosafety Level 1 (BSL 1)
• Biosafety Level 2 (BSL 2)

Field of Study

• Biochemical Sciences

Software

• Powerpoint
• NIH POTS
• Excel
• Word

Skills

• Primary cell culture
• Inventory Management
• Ordering Supplies

Disclaimer: Theabove description is meant to illustrate the generalnature of work and level of effort being performedby individuals assigned to this position or jobdescription. This is not restricted as a completelist of all skills, responsibilities, duties, and/orassignments required. Individuals may be required toperform duties outside of their position, jobdescription or responsibilities as needed.

The diversity ofAxle’s employees is a tremendous asset. We are firmlycommitted to providing equal opportunity in all aspects ofemployment and will not tolerate any illegal discriminationor harassment based on age, race, gender, religion, nationalorigin, disability, marital status, covered veteran status,sexual orientation, status with respect to publicassistance, and other characteristics protected under state,federal, or local law and to deter those who aid, abet, orinduce discrimination or coerce others to discriminate.

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As set forth in Axle’s Equal Employment Opportunity policy,we do not discriminate on the basis of any protected group status under any applicable law.

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For government reporting purposes, we ask candidates to respond to the below self-identification survey.Completion of the form is entirely voluntary. Whatever your decision, it will not be considered in the hiringprocess or thereafter. Any information that you do provide will be recorded and maintained in aconfidential file.

As set forth in Axle’s Equal Employment Opportunity policy,we do not discriminate on the basis of any protected group status under any applicable law.

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If you believe you belong to any of the categories of protected veterans listed below, please indicate by making the appropriate selection.As a government contractor subject to the Vietnam Era Veterans Readjustment Assistance Act (VEVRAA), we request this information in order to measurethe effectiveness of the outreach and positive recruitment efforts we undertake pursuant to VEVRAA. Classification of protected categoriesis as follows:

A 'disabled veteran' is one of the following: a veteran of the U.S. military, ground, naval or air service who is entitled to compensation (or who but for the receipt of military retired pay would be entitled to compensation) under laws administered by the Secretary of Veterans Affairs; or a person who was discharged or released from active duty because of a service-connected disability.

A 'recently separated veteran' means any veteran during the three-year period beginning on the date of such veteran's discharge or release from active duty in the U.S. military, ground, naval, or air service.

An 'active duty wartime or campaign badge veteran' means a veteran who served on active duty in the U.S. military, ground, naval or air service during a war, or in a campaign or expedition for which a campaign badge has been authorized under the laws administered by the Department of Defense.

An 'Armed forces service medal veteran' means a veteran who, while serving on active duty in the U.S. military, ground, naval or air service, participated in a United States military operation for which an Armed Forces service medal was awarded pursuant to Executive Order 12985.

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