Research Scientist/Engineer Assistant

Job Description

The Division of Metabolism, Endocrinology and Nutrition (MET) has an outstanding opportunity for a Research Scientist/Engineer Assistant position to join the Crisa Laboratory.

About this Opportunity

Reporting to Dr. Laura Crisa, the Research Scientist/Engineer Assistant will support studies performed and conducted in the Crisa laboratory at the University of Washington South Lake Union campus. The field of research this position is engaged in is Pancreatic islet regeneration and diabetes.

Key Responsibilities

Experiment and Assay Development (40%)

• Assist in the preparation and processing of genomic DNA, RNA, and cDNA, including support for real-time PCR workflows.
• Apply molecular biology techniques (e.g., PCR, qPCR) to analyze gene expression and identify signatures in experimental and control samples.
• Support protein-based assays, including protein extraction, pull-down assays, and gel electrophoresis.
• Perform and help optimize ELISA and metabolic assays, ensuring accuracy, reproducibility, and proper controls.
• Contribute to the development, optimization, and troubleshooting of experimental protocols.

Animal work (30%)

• Perform routine mouse handling and husbandry, including tagging, tailing, genotyping, and monitoring animal health.
• Assist with breeding colony management, including tracking, recordkeeping, and coordination of experimental cohorts.
• Ensure compliance with institutional animal care and use protocols and maintain accurate documentation.

Tissue Processing and Cell culture (20%)

• Perform immunohistochemistry and immunofluorescence staining, including tissue preparation, sectioning support, and imaging preparation.
• Maintain and perform sterile mammalian cell culture techniques, including cell plating, media changes, and basic assay support.
• Assist with tissue collection, processing, and sample preparation for downstream analyses.

Other Duties (10%)

• Maintain accurate and detailed laboratory records, including experimental data and protocols.
• Support lab operations, including purchasing, inventory management, and data entry.
• Assist with routine maintenance of laboratory equipment and ensure adequate stocking of consumables.
• Maintain a safe, organized, and collaborative laboratory environment.

Required Qualifications

To be considered for this opportunity your application must demonstrate you meet both the minimum qualifications and additional qualifications listed below. Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration.

Minimum Qualifications

• Bachelor’s degree in Biology, Biochemistry or related Scientific field and less than one year of relevant Pancreatic islet regeneration and diabetes research experience.
• Applicants who do not meet these qualifications WILL NOT be forwarded to the Hiring Manager.

Additional Qualifications

• Must be willing to work with mice.
• Hands-on experience in cell biology, molecular biology, and/or biochemistry, applied to Endocrinology and Diabetes Mellitus research.
• Demonstrated interest in pancreatic islet biology and regeneration, with a willingness to learn and apply new laboratory techniques.
• Experience with basic laboratory techniques, including pipetting, solution preparation, sterile technique, and lab safety; exposure to cell culture or molecular biology methods (e.g., PCR) preferred.
• Ability to accurately document experiments, follow established protocols, and maintain organized research records.
• Strong organizational, communication, and teamwork skills, with the ability to support multiple projects in a research setting.
• Experience with tissue culture.

Preferred Qualifications

• Prior hands-on experience with animal work including handling of large animal colonies, breeding and genetic screening of diverse mouse strains, handling and treatment of diabetic mice.
• At least 1-year hands-on experience in cell biology, molecular biology, and/or biochemistry, applied to Endocrinology and Diabetes Mellitus research.
• Experience with Metabolic Assays and protein analysis methods (e.g., Western blot, ELISA).Experience with data analysis tools (e.g., Excel, GraphPad Prism, or similar).
• Ability to quickly learn new laboratory techniques and adapt to evolving research needs.

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Compensation, Benefits and Position Details

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About the UW 

Working at the University of Washington provides a unique opportunity to change lives – on our campuses, in our state and around the world.  

UW employees bring their boundless energy, creative problem-solving skills and dedication to building stronger minds and a healthier world. In return, they enjoy outstanding benefits, opportunities for professional growth and the chance to work in an environment known for its diversity, intellectual excitement, artistic pursuits and natural beauty. 

Our Commitment 

The University of Washington is committed to fostering an inclusive, respectful and welcoming community for all. As an equal opportunity employer, the University considers applicants for employment without regard to race, color, creed, religion, national origin, citizenship, sex, pregnancy, age, marital status, sexual orientation, gender identity or expression, genetic information, disability, or veteran status consistent with UW Executive Order No. 81.

To request disability accommodation in the application process, contact the Disability Services Office at 206-543-6450 or [email protected]. 

Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law.