Research Specialist II (School of Medicine - All Children's)

We are seeking a Research Specialist II to join the lab at Johns Hopkins All Children's Hospital in St. Petersburg, Florida. This position will work in a lab focused on cancer immunology research investigating the tumor microenvironment and anti-tumor immune responses, including organoid and primary cell culture systems and in vivo preclinical tumor models. The specialist will contribute to immunotherapy and translational research studies within a collaborative and dynamic laboratory environment.

The Research Specialist II develops assays and protocols for PI approval and designs experimental approaches for laboratory projects. Performs a range of sophisticated assays. Makes significant contributions to various aspects of laboratory efforts and goals. Responsible for troubleshooting, independent decisions and training/guidance of other laboratory personnel.

Specific Duties & Responsibilities

• Conduct assigned laboratory projects.
• Utilize specialized knowledge to interpret results, e.g. read controls, evaluate results, etc.
• Work independently and use scientific knowledge and lab experience to make judgments and decisions.
• Teach/train learners, and new technicians in the laboratory.
• Guide, direct, and facilitate problem solving for entry-level laboratory members on day-to-day problems with more challenging research assays.
• Make scientific contributions to protocol development, efficiency, and productivity.
• Collaborate in the development of new techniques and instruments.
• Apply published research methods to current experimental work by investigating published protocols and discussing them with PI.
• Scientifically contribute to the preparation of manuscripts and proposals.
• Process analyzed data into figures for scientific publications.
• Order equipment and supplies, interface with vendors.
• Maintain daily records of experiments.
• Organize and track laboratory samples.
• Other duties as assigned.

• Bachelor's Degree in Biology, Chemistry or related field.
• Two years of laboratory experience performing relevant techniques including experimental techniques used in genomics, sequencing, and cancer biology assays.
• Additional education may substitute for required experience and additional related experience may substitute for required education beyond a high school diploma/graduation equivalent, to the extent permitted by the JHU equivalency formula.

• Master's Degree with related graduate research.
• Cell culture.
• RNA isolation and quantitative PCR (qPCR).
• Common cell biology assays.
• Genomics and sequencing.
• Immunostaining.
• Western blotting.
• FACS analysis.
• Mouse handling.
• Bioinformatics analysis.
• GraphPad/Prism/Adobe.

Technical Skills & Expected Level of Proficiency

• Assay Development and Validation - Developing
• Data Management and Analysis - Developing
• Experimental Design and Execution - Developing
• Operations Management - Developing
• Protocol Development and Optimization - Developing
• Scientific Writing - Developing
• Stakeholder Engagement - Developing

The core technical skills listed are most essential; additional technical skills may be required based on specific division or department needs.

Classified Title: Research Specialist II   
Role/Level/Range: ACRP/03/MB  
Starting Salary Range: $42,330 - $74,110 Annually ($55,650 targeted; Commensurate w/exp.) 
Employee group: Full Time 
Schedule: Mon-Fri, 8.30am-5.00pm 
FLSA Status: Exempt 
Location: Florida  
Department name: All Childrens   
Personnel area: School of Medicine